The antioxidative properties of a novel curcumin analogue (2models, including superoxide anion, hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and PC12 cell protection from H2O2 damage. used in traditional Indian and Chinese medicine for the treatment of many diseases including inflammation, dyspesia, respiratory disorders, arthritis and others [1]. Curcumin has exhibited 915191-42-3 IC50 diverse pharmacological activities such as anti-carcinogenic, anti-inflammatory, antioxidant and antimicrobial activities [2]. Furthermore, some reports have suggested possible beneficial effects of curcumin on the animal models and human studies of Rabbit Polyclonal to DRD4 Alzheimers disease [3]. Physique 1. Chemical structure of curcumin and synthesis of (2means absorption of the answer assessed at 410 nm. Data presented are … 2.3. Cytotoxicity of MCH in PC12 Cells After 24 h treatment with MCH at concentrations between 0.63 and 5 M, the reduction in cell viability was no greater than 9% (Physique 3). At the highest concentration (30 M) evaluated, the reduction in cell viability was 21.8%. There is usually no significant difference in cytotoxicity induced by MCH and curcumin in PC12 cells. Physique 3. Effects of MCH and curcumin on PC12 cells. Cells were treated with 0.63C30 M MCH or curcumin for 24 h. Data presented are the means SD of results from three impartial experiments. 2.4. MCH Protects PC12 Cells against H2O2-Induced Cytotoxicity Compared with normal PC12 cells, cells uncovered to 150 M H2O2 for 3 915191-42-3 IC50 h exhibited morphological alteration, including a designated decrease in cell number, cell shrinkage and membrane blebbing (Physique 4A). The pretreatment of 5 M MCH or 10 M curcumin could mitigate such cell damages. As estimated by MTT 915191-42-3 IC50 assay, cell viability was markedly decreased to 46.2% after a 3 h exposure to 150 M H2O2. However, when cells were pre-incubated with MCH (0.63C10.00 M) for 30 min, cell toxicity was significantly attenuated in a dose-dependent manner (Physique 4B). Pretreatment of PC12 cells with MCH (0.63C10.00 M) and 10 M curcumin for 30 min significantly elevated the cell viability of PC12 cells to a range of 60.9%C75.4% and 72.7%, respectively. A 50% reduction in H2O2-induced cell death (H2O2-only treatment), respectively. Physique 10. Effects of MCH on the manifestation of Nrf2 genes. PC12 cells were treated for 24 h with 150 M H2O2 in the absence/presence of 5 M of Cur/MCH pretreatment (30 min). The manifestation of Nrf2 was decided by semi-quantitative RT-PCR. GAPDH … 3.?Discussion Curcumin, a natural phenolic diarylheptanoid was reported to have neuroprotective effects via reducing oxidative stress [3]. The potent chain-breaking antioxidant activity of curcumin has currently received amazing interest for its common radical trapping ability [19]. Although a lot of work has been reported on the potential use of curcumin as an antioxidant, the search for new derivatives or analogues is usually ongoing to develop compounds that have a better antioxidant activity [5]. In our present study, a novel curcumin analogue MCH, proved effective in superoxide anion scavenging and PC12 cell protection from oxidative damage. Although MCH was less potent than curcumin in scavenging capacity on DPPH and hydroxyl radical by chemical reaction (Table 1), it markedly reduced the ROS levels in PC12 cells after the pretreatment at 0.63C5.00 M. In addition to possible direct free radical scavenging, MCH may have indirect effects, such as the modulation of endogenous antioxidant enzymes to reduce ROS levels. Among the most important defenses against oxygen radicals are CAT and 915191-42-3 IC50 SOD enzymes. SOD catalyses dismutation of the superoxide anion into H2O2 and CAT 915191-42-3 IC50 detoxifies H2O2 to oxygen and water [20]. The combined action of these two enzymes reduces ROS levels in cells and repairs oxidized.