Supplementary MaterialsFigure S1: Rapamycin destabilizes the mTOR-raptor/eIF3f interaction. 2-3 times in the presence of 100nM insulin. Cell lysates were prepared and analyzed by immunoblotting.(5.38 MB EPS) pone.0008994.s003.eps (5.1M) GUID:?6329CD93-67B4-4172-BE7C-0E96921EF3CC Number S4: Specific down regulation of eIF3f expression by ShRNAi. The small interfering RNA (ShRNAi) studies used oligonucleotide complementary RNA with symmetrical two nucleotide overhangs which… Continue reading Supplementary MaterialsFigure S1: Rapamycin destabilizes the mTOR-raptor/eIF3f interaction. 2-3 times in