The NADPH-dependent carbonyl reductase S1 from stereoselectively catalyzes the reduced amount of ethyl 4-chloro-3-oxobutanoate (COBE) to ethyl (and purified by Ni-affinity ion-exchange and size-exclusion chromatography. a novel enzyme that produces (Rosetta (DE3) (Novagen) cells were transformed with the expression vector constructed above and the recombinant cells were cultured in 1?l LB medium containing 10?μg?ml?1 kanamycin… Continue reading The NADPH-dependent carbonyl reductase S1 from stereoselectively catalyzes the reduced amount
Tag: ASA404
Latent TGFβ-binding proteins 1 (LTBP-1) is an integral regulator of TGFβ
Latent TGFβ-binding proteins 1 (LTBP-1) is an integral regulator of TGFβ targeting and activation in the extracellular matrix. we’ve cloned the mouse gene promoter and analysed its system of transcriptional repression by AhR. Reporter gene assays AhR over-expression and site-directed mutagenesis demonstrated that basal transcription can be AhR-dependent. Chromatin immunoprecipitation (ChIP) and RNA disturbance (RNAi)… Continue reading Latent TGFβ-binding proteins 1 (LTBP-1) is an integral regulator of TGFβ