Selective 15N isotope labeling of the cytochrome with auxotrophs was utilized to characterize the hyperfine couplings using the side-chain nitrogens from R71 H98 and Q101 residues and peptide nitrogens from R71 and H98 residues across the semiquinone (SQ) in the high-affinity QH site. a lot of the unpaired spin denseness rather than the Nε of… Continue reading Selective 15N isotope labeling of the cytochrome with auxotrophs was utilized